|
KMID : 0616120170480020125
|
|
Korean Journal of Pharmacognosy
2017 Volume.48 No. 2 p.125 ~ p.133
|
|
|
Antioxidative and Cellular Protective Effects of Dolwoe (Gynostemma pentaphyllum Makino) Extracts against Oxidative Stress
|
|
Kim Kyoung-Mi
Kim A-Rang
Kim A-Young
Ha Ji-Hoon
Xuan Song Hua
Jeong Yoon-Ju
Park Young-Min
Jeong Hyo-Jin
Hong In-Gi
Park Soo-Nam
|
|
|
Abstract
|
|
|
|
In this study, we investigated the total phenolic and flavonoid contents, component analysis, antioxidative activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Gynostemma pentaphyllum (G. pentaphyllum) Makino. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites (FSC50) of the 50% ethanol extracts, ethyl acetate fraction and aglycone fraction were 246.8, 147.2, 128.9 μg/mL, respectively. Reactive oxygen species (ROS) scavenging activities (OSC50) of the 50% ethanol extract, ethyl acetate fraction and aglycone fraction on ROS generated in Fe 3+-EDTA/H2O2 system using the luminol-dependent chemiluminescence assay were 37.15, 10.74, 7.19 μg/mL, respectively. We investigated the cellular protective activity and the results showed that treatment of aglycone fraction (0.05-0.39 μg/mL) protect human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with H2O2. These results suggest that extract/fractions of G. pentaphyllum Makino may be applicable as natural antioxidants in cosmetics.
|
|
|
KEYWORD
|
|
|
Gynostemma pentaphyllum Makino, Antioxidant, Cellular protective effect, Components analysis, Cosmetics
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|